View ORCID ProfilePauline Durand-Smet, Tamsin A. Spelman, View ORCID ProfileElliot M. Meyerowitz, and also View ORCID ProfileHenrik Jönsson
steustatiushistory.org July 21, 2020 117 (29) 17399-17408; first publimelted July 8, 2020; https://doi.org/10.1073/steustatiushistory.org.2003184117
bDivision of Biology and also Biological Engineering, California Institute of Technology, Pasadena, CA 91125;
dDepartment of Applied Mathematics and Theoretical Physics, University of Cambridge, Cambridge CB3 0WA, United Kingdom;
eDepartment of Astronomy and Theoretical Physics, Computational Biology and also Biological Physics, Lund University, 221 00 Lund, Sweden

Contributed by Elliot M. Meyerowitz, May 19, 2020 (sent out for review February 20, 2020; reperceived by Naomi Nakayama and Timothy E. Saunders)


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Significance

The cytoskeleton, a netjob-related of polymers including microtubules and also actin, supports many type of functions in cells. In plants, the cytoskeleton orientation is a critical parameter dictating the direction of cell expansion. While light, hormonal, or mechanical signals deserve to influence the cytoskeleton organization, the role of cell geomeattempt stays to be clarified. With a microwell-based method, we confined plant cells doing not have walls in various geometries and also discovered that the cytoskeletons align via the long axis in cells in rectangular wells. Basic geometrical rules of the microtubules are computationally modeled in 3 dimensions and also disclose the role of severing proteins in the form response, which was oboffered experimentally. These findings show exactly how cell geomeattempt feeds ago on cytoskeletal organization in plant cells.

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Abstract

The cytoskeleton plays a key function in developing robust cell form. In pets, it is well established that cell shape deserve to likewise influence cytoskeletal organization. Cytoskeletal proteins are well conoffered in between animal and also plant kingdoms; nevertheless, because plant cells exhilittle bit significant structural differences to animal cells, the question arises whether the plant cytoskeleton likewise responds to geometrical cues. Recent numerical simulations predicted that a geometry-based dominion is adequate to describe the microtubule (MT) organization oboffered in cells. Due to their high flexural rigidity and also persistence length of the order of a few millimeters, MTs are rigid over cellular dimensions and are for this reason meant to align along their lengthy axis if constrained in certain geometries. This hypothesis remains to be tested in cellulo. Here, we discover the family member contribution of geometry to the last organization of actin and MT cytoskeleloads in single plant cells of Arabidopsis thaliana. We present that the cytoskeleton aligns via the long axis of the cells. We uncover that actin organization depends on MTs but not the opposite. We develop a design of self-arranging MTs in 3 dimensions, which predicts the importance of MT severing, which we confirm experimentally. This occupational is an initial action toward assessing quantitatively how cellular geometry contributes to the regulate of cytoskeletal company in living plant cells.


How organisms achieve their particular develops and also alter their growth trends in response to environmental signals has been the topic of examination for over a century (1). The prominence of shape for specific organic attributes has actually been shown across kingdoms (2⇓–4). As an instance, the aperture and also closure system of pores allowing gas exadjust in between plants and also the environment count on the form of stomatal guard cells in leaves of flowering plants (3, 5, 6). Anvarious other instance is that the elongation of cells promotes a prohealing phenoform in macropheras (4).

The cytoskeleton, an interassociated netoccupational of filamentous polymers such as actin and microtubules (MTs), plays a key duty in developing robust cell form. In pet cells, the actomyosin cortex offers the cells their mechanical stamina and acts in regulating their forms (7⇓⇓–10). In plant cells, a cortical MT network, rather than actin, prescribes the form of cells by guiding the synthesis of cellulose in the cell wall (11) and also supports the mechanical stamina of plant protoplasts (plant cells with their wall surfaces enzymatically removed) (12). In growing plant cells, the actin netjob-related is likewise connected in cell wall formation by being responsible for the global distribution of cellulose synthase complexes at the plasma membrane (13). The cytoskeleton is thus a major determinant of cell form. In revolve, cell geomeattempt has been presented to impact cytoskeletal company in pet cells (14⇓⇓⇓–18). Moreover, a geometrical restriction is recognized to alter nuclear morphology and chromatin compaction (19, 20), highlighting the influence of cell geometry in modulating transcriptional response. In plants, numerous exterior components have actually been presented to affect MT cytoskeletal company such as light, hormones (21), and also mechanical stresses (22⇓–24). The evaluation of the role of geometric cues in managing cytoskeleton in planta is doing not have and also stays to be quantified. Cytoskeletal company in animal and also plant cell kinds is rather different. While the mechanical properties of animal cells and also their shape integrity depend mainly on a thick network of cortical actin, interphase MTs are much less numerous and also are connected in intracellular vesicle trafficking during interphase (25, 26). Conversely, plant cells screen a dense netjob-related of cortical MTs that is connected in cell wall synthesis (11, 27). The actin cytoskeleton in plant cells is largely uncovered within more main areas of the cytoplasm, notably within cytoplasmic strands that cross the vacuole and also affix to the nucleus (28). In enhancement, plant cells absence centrosomes, which leads to diffuse MT nucleation within the cell (SI Appendix, Fig. S1). Although cytoskeletal proteins are well conserved in eukaryotes, the distinctions in company raise the question of whether cytoskeletal response to geometrical cues is conserved between plants and also animals.

In planta, various MT organizations have the right to be oboffered in cells via similar geometry within the same tworry, for instance in epidermal areas within the shoot apical meristem or in various cell layers in the hypocotyl of Arabidopsis thaliana plants. Tissue shape-acquired mechanical stresses were shown to play a dominant role in organizing the MTs in these cases (23, 24). Since of the coupling between mechanical stress and also tissue geomeattempt, the duty of cell geomeattempt alone on cytoskeletal organization can be difficult to estimate when working at the tworry level. Due to their high flexural rigidity and also persistence size of the order of a couple of millimeters (29), MTs are rigid over cellular dimensions and also have the right to be intended to align along their lengthy axes if constrained in certain geometries. However, many kind of models predicted that MT–MT interactions influence the capacity of alignment (30⇓⇓⇓–34), and also the alignment along the longest axis hypothesis stays to be tested in cellulo. Specifically, the severing of MTs at crossing sites by the protein katanin (35⇓⇓–38) has actually been proposed to promote MT alignment. Recently, an extensive theoretical examine of the impact of severing of MTs highlighted the prominence of taking right into account this dynamical home in two-dimensional (2D) simulations (39). The MT company that emerges from 3D simulations once incorporating the severing rule, and whether that coincides through experimental monitorings, is not clear. Recent in vitro experiments through semiversatile filament options (40⇓⇓–43) and three-dimensional (3D) models in embryonic plant cells (44⇓–46) predicted that a geometry-based ascendancy is enough to describe the MT company. However before, this version has not been empirically tested.

It is recognized that actin organization is influenced by mechanical pressures in pet cells (47⇓–49), but exactly how actin filaments respond to mechanical stresses has actually rarely been investigated in plants (50⇓–52). Due to the fact that the actin filaments and MTs have actually been shown to situate near the plasma membrane in plant cells, it has actually been suggested that the 2 networks interact (53). The specific interactions in between actin and MTs are poorly construed, however one study demonstrated that actin filament reassembly depends on MTs (54), probably via formin interactions (54⇓–56). Whether actin filaments and MTs behave actually similarly or connect in the context of an answer to geometrical or mechanical perturbation still stays to be establimelted.

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In this study, we emerged an speculative approach to discover the contribution of geomeattempt to the final company of actin and MT cytoskelelots in isolated plant cells doing not have cell walls. We extended a formerly occurred model of the 3D self-organizing MT netjob-related to predict the role of severing MTs on the shape response. We show that geomeattempt alone is enough to describe the oboffered reorganization of MTs in rectangular shapes and also that the geomeattempt response is dependent on severing. In addition, studying actin and MTs in the very same system permitted us to compare the organization of the two networks, and just how they interact. We show that the actin response to form change relies on the MT network but not vice versa.