You are watching: Why do you suppose the acid fast stain is not as widely used
How does heating the bacterial smear throughout a ZN stain promote entry of carbolfushin right into the acid-quick cell wall?
Yes, they are stained initially. However, the decolorizer takes amethod the shade on the acid-quick negative however not the acid fast positive bacteria.
Why is the acid rapid stain not as commonly provided as the gram stain? When is it more advantageous than the gram stain?
incredibly few bacteria are acid quick positive, so the test is much less useful than a gram stain, which separates organisms into 2 large teams. -acid quick is beneficial as soon as acid rapid positive bacteria are suspected
diluted microbial sample is deposited on an agar plate and spreview uniformly throughout the surconfront with a glass rodvia a effectively diluted sample, cells will certainly be deposited far enough apart on the agar surconfront to prosper right into individual colonies
a special condenser is provided so only the light reflected off the specimen enters the objective-dark background
acidic stain; include bacteria to one drop of stain on slide and also use one more slide to spread the black ink throughout the slide-perform not heat fix
Phenylethyl alcohol agaruncharacterized, selective media-inhibits gram negative growth-results = poor or great growth
selective (gram+), differential (mannitol)7.5% NaCl + phenol red-poor/no development = not staph-good development = staph-yellow = mannitol fermentation-red = does not ferment mannitol
selective (gram-), differential (for lactose)media-capability to ferment lactose-poor/no growth = gram positive-good expansion = gram negative-pink/red = lactose fermentation, coliform-colorless = no reaction/fermentation
selective (gram -), differential (lactose, sucrose) dyes inhibit expansion of gram positive organisms and react via lactose fermenters-poor/no development = gram positive-good development = gram negative-pink = ferments lactose, little acid production-purple/black with/out green sheen = ferments lactose and/or sucrose with acid production-colorless = no lactose/sucrose fermentation (noncoliform)
differential-yellow + bubble = fermentation with gas-yellow, no bubble = fermentation w/out gas-red, no bubble = no fermentation-pink, no bubble = deterioration of peptone, alkaline
uses same media s VP test-detects organisms qualified of perdeveloping blended acid fermentation-supplies a methyl red indicator complying with incubation-red = blended acid fermentation-no color = no blended acid fermentation
provides exact same media as methyl red test-adding VP reagents oxidizes acetoin and diacetyl-red = acetoin produced-no color = no acetoin
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